My singapore Idol!

My singapore Idol!

Sunday, December 13, 2009

Why am i in school on a sunday?

As the title suggests, probably u can guess why i end up in school on a sunday. I have to grow cells before i can start the expt on monday. If not, I would waste another day again. But luckily I did. I was shocked when i came back to lab and only realise that my medium is contaminated with cells! I was at a loss to what i can do because i need the medium to grow the cells. I thought of various ways to solve it but none of it met my expectation. I planned to change my schedule but I am doubtful that it would be good. Sigh. Luckily my savior came in, postdoc! He came back with the autoclave keys and i am very thankful of him for helping me to find it. Savior! haha. So, i can progress smoothly; 6pm innoculate cells and 10pm innoculate another batch of cells =)
Still, not many people work on sunday and I feel kind of lonely. haha.

Saturday, December 5, 2009

Should I or should I not?

haha. Yesterday's meeting was funny. I shouldnt have prepared something for my presentation because my exam ended on wednesday and i think my sup didnt expect me to do anything much on thursday either and so, he skipped my turn and went into his fav opening line for end of presentation " Any lab matters to discuss?" Perhaps my senior saw that i uploaded ppt slides on the google docs and she said "mh has done something for the presentation." I quickly said nvm dont need. Then sup was saying "really? ok". Then yah i presented. Actually during the postdoc presentation he mention about the project being splitted up by my senior and me. Then boss was saying "mh are u ready?". of coz i said " erm...yes". But my thoughts were "Thought I already started straight after exam?hmm?". After he was surprised i prepared sth for the presentation then i know why he is shocked, coz he nv thought that i was so 'eager' to do lab straight after exam. haha. I scalded my index finger for nth.

I failed my expt on friday but i found out the reason for the failure and the postdoc recommended me a solution and yep. Of coz i was happy. My schedule was to complete the assay on friday and monday can start measuring the activity of supernatant of cells. But the problem is, I dont know where are the strains. Then i need to get single colony which takes 2 days and then overnight dilution which takes another day. then i cant start on monday !!!!! If I can get agar plates with these strains from my senior, then i can fit into my schedule perfectly. The problem is sunday i must come back no matter wat. 1st is to prepare the stuff (innoculate cells) and then to synthesise AI-2 (because i keep diluting, and try whatever methods that come to my mind to improve my results and finish up the AI-2 that i synthesised on thursday) for re-doing luminesecence assay. I have to finish watever task that was allocated to me by next week!!!! (I havent go through 1st trail yet, being inexperienced I scared that I may make a mistake along the way)